effect of harmine alkaloid on the expression of p16 and dapk in hl60 leukemia cell line
نویسندگان
چکیده
introduction: epigenetic changes such as promoter methylation of tumor suppressor genes (tsgs) is one of the most important mechanisms involved in the development of hematologic malignancies. harmine is one of the harmal-derived alkaloids with anti-proliferatory effects on leukemia cell lines. since p16 and dapk genes are hypermethylated in some hematologic malignancies, the current study aimed to investigate the effect of harmine on the expression of these two genes in hl60 leukemia cell line to take steps towards clarifying the mechanism of it’s anti-proliferatory effect. methods and materials: hl60 cells were seeded into 96 well plates containing rpmi medium supplemented with 10% fbs, 1% penicillin or streptomycin, and then incubated at 37°c in a humid environment at 5% co2. cell count and viability were determined after 24, 48, and 72 hours in the presence of harmine and 5-azacytidine. gene expression analysis was performed by quantitative real time pcr. finally, statistical analysis was carried out using spss software. results: based on the results, harmine suppresses cell proliferation in all concentrations. comparing the control group, harmine at 25.6 µg/ml and 102.4 µg/ml concentrations reduces cell proliferation to 50 and 78 percent at 72 hours, respectively (p<0.001). the results demonstrate that harmine at 102.4 µg/ml concentration significantly upregulates dapk expression (p<0.005). however, its effect was not significant on p16 expression (p>0.05). discussin and conclusion: the results indicate that harmine have inhibitory effect on hl60 leukemia cell line in a dose and time-dependent manner. furthermore, harmine can induce dapk upregulation that might be related to dapk gene hypomethylation. further comprehensive and elucidative investigations are needed for better understanding of the harmine effect on leukemic cells.
منابع مشابه
بررسی بیان پروتئینهای P16 و DAPK در ردهی سلولی لوسمیک HL60 در مجاورت با آلکالوئید گیاهی
Introduction: Epigenetic changes such as promoter methylation of tumor suppressor genes (TSGs) is one of the most important mechanisms involved in the development of hematologic malignancies. Harmine is one of the Harmal-derived alkaloids with anti-proliferatory effects on leukemia cell lines. Since P16 and DAPK genes are hypermethylated in some hematologic malignancies, the current study aimed...
متن کاملthe effect of explicit teaching of metacognitive vocabulary learning strategies on recall and retention of idioms
چکیده ندارد.
15 صفحه اولthe washback effect of discretepoint vs. integrative tests on the retention of content in knowledge tests
در این پایان نامه تاثیر دو نوع تست جزیی نگر و کلی نگر بر به یادسپاری محتوا ارزیابی شده که نتایج نشان دهندهکارایی تستهای کلی نگر بیشتر از سایر آزمونها است
15 صفحه اولthe effect of taftan pozzolan on the compressive strength of concrete in the environmental conditions of oman sea (chabahar port)
cement is an essential ingredient in the concrete buildings. for production of cement considerable amount of fossil fuel and electrical energy is consumed. on the other hand for generating one tone of portland cement, nearly one ton of carbon dioxide is released. it shows that 7 percent of the total released carbon dioxide in the world relates to the cement industry. considering ecological issu...
: the effect of sericin levels (silk glue protein) on rate of in vitro maturation, fertilization and culture of sheep oocytes
هدف از آزمایش اول بررسی اثر سطوح مختلف سریسین [0 (control), 0.1, 0.5, 1.0, 2.5 %] افزوده شده به محیط , ivm بر cumulus cell expansion، بلوغ هسته و توسعه متوالی جنین، در گوسفندان نژاد سنجابی در فصل تولید مثلی می باشد. از سرگیری میوز به وسیله خارج شدن اولین پولار بادی اندازه گیری و هم چنین درصد رسیدن جنین های دو سلولی به مرحله کلیواژ و بلاستوسیت نیز به عنوان نشانه ای از میزان شایستگی توسعه اولیه ج...
the effect of e-64 on the developmental competence of sheep cocs during in vitro maturation
in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.
منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
علوم پیراپزشکی و بهداشت نظامیجلد ۱۱، شماره ۳، صفحات ۲۸-۳۳
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023